Tools & Reagents
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Tools & Reagents |
| Vector | Origin of replication | Resistance | Promoter | Cloning site |
Data sheet (.pdf) |
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| pZS*13luc | pSC101* | Ampicillin | PA1lacO-1 | luciferase | pZS*13luc.pdf | |||||
| pZS*21MCS | pSC101* | Kanamycin | PLtetO-1 | MCS | pZS*21MCS.pdf | |||||
| pZS*21luc | pSC101* | Kanamycin | PLtetO-1 | luciferase | pZS*21luc.pdf | |||||
| pZS*24MCS | pSC101* | Kanamycin | Plac/ara-1 | MCS | pZS*24MCS.pdf | |||||
| pZS*24luc | pSC101* | Kanamycin | Plac/ara-1 | luciferase | pZS*24luc.pdf | |||||
| pZS13luc | pSC101 | Ampicillin | PA1lacO-1 | luciferase | pZS13luc.pdf | |||||
| pZS21MCS | pSC101 | Kanamycin | PLtetO-1 | MCS | pZS21MCS.pdf | |||||
| pZS21luc | pSC101 | Kanamycin | PLtetO-1 | luciferase | pZS21luc.pdf | |||||
| pZA11MCS | p15A | Ampicillin | PLtetO-1 | MCS | pZA11MCS.pdf | |||||
| pZA13luc | p15A | Ampicillin | PA1lacO-1 | luciferase | pZA13luc.pdf | |||||
| pZA14luc | p15A | Ampicillin | Plac/ara-1 | luciferase | pZA14luc.pdf | |||||
| pZA21MCS | p15A | Kanamycin | PLtetO-1 | MCS | pZA21MCS.pdf | |||||
| pZA23MCS | p15A | Kanamycin | PA1lacO-1 | MCS | pZA23MCS.pdf | |||||
| pZA24luc | p15A | Kanamycin | Plac/ara-1 | luciferase | pZA24luc.pdf | |||||
| pZA31MCS | p15A | Chloramphenicol | PLtetO-1 | MCS | pZA31MCS.pdf | |||||
| pZA31luc | p15A | Chloramphenicol | PLtetO-1 | luciferase | pZA31luc.pdf | |||||
| pZA33luc | p15A | Chloramphenicol | PA1lacO-1 | luciferase | pZA33luc.pdf | |||||
| pZA34luc | p15A | Chloramphenicol | Plac/ara-1 | luciferase | pZA34luc.pdf | |||||
| pZA35luc | p15A | Chloramphenicol | PN25tetO-1 | luciferase | pZA35luc.pdf | |||||
| pZE11luc | colE1 | Ampicillin | PLtetO-1 | luciferase | pZE11luc.pdf | |||||
| pZE12MCS | colE1 | Ampicillin | PLlacO-1 | MCS | pZE12MCS.pdf | |||||
| pZE12luc | colE1 | Ampicillin | PLlacO-1 | luciferase | pZE12luc.pdf | |||||
| pZE13MCS | colE1 | Ampicillin | PA1lacO-1 | MCS | pZE13MCS.pdf | |||||
| pZE13luc | colE1 | Ampicillin | PA1lacO-1 | luciferase | pZE13luc.pdf | |||||
| pZE14MCS | colE1 | Ampicillin | Plac/ara-1 | MCS | pZE14MCS.pdf | |||||
| pZE15luc | colE1 | Ampicillin | PN25tetO-1 | luciferase | pZE15luc.pdf | |||||
| pZE21MCS | colE1 | Kanamycin | PLtetO-1 | MCS | pZE21MCS.pdf | |||||
| pZE21luc | colE1 | Kanamycin | PLtetO-1 | luciferase | pZE21luc.pdf | |||||
| pZE22luc | colE1 | Kanamycin | PLlacO-1 | luciferase | pZE22luc.pdf | |||||
| pZE23MCS | colE1 | Kanamycin | PA1lacO-1 | MCS | pZE23MCS.pdf | |||||
| pZE23luc | colE1 | Kanamycin | PA1lacO-1 | luciferase | pZE23luc.pdf | |||||
| pZE24MCS | colE1 | Kanamycin | Plac/ara-1 | MCS | pZE24MCS.pdf | |||||
| pZE24luc | colE1 | Kanamycin | Plac/ara-1 | luciferase | pZE24luc.pdf | |||||
| pZE25luc | colE1 | Kanamycin | PN25tetO-1 | luciferase | pZE25luc.pdf | |||||
| pZE31luc | colE1 | Chloramphenicol | PLtetO-1 | luciferase | pZE31luc.pdf | |||||
| pZE32luc | colE1 | Chloramphenicol | PLlacO-1 | luciferase | pZE32luc.pdf | |||||
| pZE33luc | colE1 | Chloramphenicol | PA1lacO-1 | luciferase | pZE33luc.pdf | |||||
| pZE34MCS | colE1 | Chloramphenicol | Plac/ara-1 | MCS | pZE34MCS.pdf | |||||
| pZE53luc | colE1 | Tetracycline | PA1lacO-1 | luciferase | pZE53luc.pdf |
| Vector | Origin of replication | Resistance | Promoter | Cloning site |
Map/ Sequence (.pdf) |
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| pZS4Int-laci | pSC101 | Spectinomycin | -- | -- | pZS4Int-laci.pdf | |||||
| pZS4Int-tetR | pSC101 | Spectinomycin | -- | -- | pZS4Int-tetR.pdf | |||||
| pZS4Int-laci/tetR | pSC101 | Spectinomycin | -- | -- | pZS4Int-laci/tetR.pdf |
| Strain | Genotype | Main features | ||
DH5alphaZi |
laciq, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1 |
Its high transformation efficiency makes DH5alpha suited for DNA cloning and DNA preparation. Due to its relatively low growth rates it is not widely used for large scale protein production (e.g. by fermentation) |
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| DH5alphaZ1 | laciq, PN25-tetR, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1 |
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| C600Z1 |
laciq, PN25-tetR, SpR, lacY1, leuB6, mcrB+, supE44, thi-1, thr-1, tonA21 |
A high transformation efficiency combined with extremly fast growth rates makes C600Z1 a superb candidate for DNA cloning as well as high level protein production. RecA mediated chromosomal integration of DNA-sequences and general transduction, e.g. by P1, is also feasible. |
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| C600Zi |
laciq, SpR, lacY1, leuB6, mcrB+, supE44, thi-1, thr-1, tonA21 |
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| W3110Z1 |
laciq, PN25-tetR, SpR, IN (rrnD-rrnE)1, rph-1 (see: ATCC 39936) |
W3110 is widely used in large scale protein production processes (fermentation). Due to its low transformation efficiency it is not suited for DNA cloning. |
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| Kit | Purpose of use | System components | Main features |
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| pZ Lac Basic |
Standard DNA cloning. High-level expression of recombinant proteins. Protein purification. |
Cloning and Expression Vectors: pZE13MCS (pZE13MCS.pdf) Control Expression Vector: pZPlac E. coli strain C600Zi |
Vectors contain combinations of the following modules: • Expression module: IPTG-inducible promoter PA1lacO-1 in combination with either MCS or luciferase • Resistance module: Ampicillin • Replication module: colE1 Host strain C600Zi produces constitutively chromosomally encoded Lac Repressor |
| pZ Tet Basic |
Standard DNA cloning. High-level expression of recombinant proteins. Protein purification. |
Cloning and Expression Vectors: pZE15luc (pZE15luc.pdf) Control Expression Vector: pZPtet E. coli strain C600Z1 |
Vectors contain com binations of the following modules: • Expression module: Tetracycline-inducible promoter PN25tetO-1 in combination with luciferase • Resistance module: Ampicillin • Replication module: colE1 Host strain C600Z1 produces constitutively chromosomally encoded Lac Repressor and Tet Repressor |
| pZ High Performance |
Quantitative and independent control of up to three different transcription units. Cloning and expression of toxic genes and gene products. Functional analysis of low copy number proteins where a broad window of regulation (up to 80,000 fold) and/or graded expression is required. High-level expression of recombinant proteins |
Cloning and Expression Vectors: pZE21MCS (pZE21mcs.pdf) pZA31MCS (pZA31mcs.pdf) pZE12luc (pZE12luc.pdf) pZE53luc (pZE53luc.pdf) pZS*24MCS (pZS*24mcs.pdf) Control Expression vectors: pZPtet and pZPlac E. coli strains: DH5alphaZ1 W3110Z1 |
Vectors contain combinations of the following modules: • Expression modules: IPTG-inducible promoters PA1lacO-1 and PLlacO-1 IPTG/Arabinose-inducible promoter Plac/ara-1 , Doxycycline-inducible promoter PLtetO-1 combined with either an MCS or luciferase. • Resistance modules: Kanamycin Chloramphenicol Tetracycline • Replication modules: colE1 p15A pSC101* Vector modules are largely interchangeable by unique restriction sites and compatible with pZ BASIC. Host strain DH5alphaZ1 and W3110Z1 produce constitutively chromosomally encoded LacR, TetR, and AraC. |