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Tools & Reagents



The following components of the pZ Expression System are available:

1 pZ Vectors

1.1 Vectors for Cloning and Expression

Vector Origin of replication Resistance Promoter Cloning site
Data sheet (.pdf)

pZS*13luc pSC101* Ampicillin PA1lacO-1 luciferase pZS*13luc.pdf
pZS*21MCS pSC101* Kanamycin PLtetO-1 MCS pZS*21MCS.pdf
pZS*21luc pSC101* Kanamycin PLtetO-1 luciferase pZS*21luc.pdf
pZS*24MCS pSC101* Kanamycin Plac/ara-1 MCS pZS*24MCS.pdf
pZS*24luc pSC101* Kanamycin Plac/ara-1 luciferase pZS*24luc.pdf
pZS13luc pSC101 Ampicillin PA1lacO-1 luciferase pZS13luc.pdf
pZS21MCS pSC101 Kanamycin PLtetO-1 MCS pZS21MCS.pdf
pZS21luc pSC101 Kanamycin PLtetO-1 luciferase pZS21luc.pdf
pZA11MCS p15A Ampicillin PLtetO-1 MCS pZA11MCS.pdf
pZA13luc p15A Ampicillin PA1lacO-1 luciferase pZA13luc.pdf
pZA14luc p15A Ampicillin Plac/ara-1 luciferase pZA14luc.pdf
pZA21MCS p15A Kanamycin PLtetO-1 MCS pZA21MCS.pdf
pZA23MCS p15A Kanamycin PA1lacO-1 MCS pZA23MCS.pdf
pZA24luc p15A Kanamycin Plac/ara-1 luciferase pZA24luc.pdf
pZA31MCS p15A Chloramphenicol PLtetO-1 MCS pZA31MCS.pdf
pZA31luc p15A Chloramphenicol PLtetO-1 luciferase pZA31luc.pdf
pZA33luc p15A Chloramphenicol PA1lacO-1 luciferase pZA33luc.pdf
pZA34luc p15A Chloramphenicol Plac/ara-1 luciferase pZA34luc.pdf
pZA35luc p15A Chloramphenicol PN25tetO-1 luciferase pZA35luc.pdf
pZE11luc colE1 Ampicillin PLtetO-1 luciferase pZE11luc.pdf
pZE12MCS colE1 Ampicillin PLlacO-1 MCS pZE12MCS.pdf
pZE12luc colE1 Ampicillin PLlacO-1 luciferase pZE12luc.pdf
pZE13MCS colE1 Ampicillin PA1lacO-1 MCS pZE13MCS.pdf
pZE13luc colE1 Ampicillin PA1lacO-1 luciferase pZE13luc.pdf
pZE14MCS colE1 Ampicillin Plac/ara-1 MCS pZE14MCS.pdf
pZE15luc colE1 Ampicillin PN25tetO-1 luciferase pZE15luc.pdf
pZE21MCS colE1 Kanamycin PLtetO-1 MCS pZE21MCS.pdf
pZE21luc colE1 Kanamycin PLtetO-1 luciferase pZE21luc.pdf
pZE22luc colE1 Kanamycin PLlacO-1 luciferase pZE22luc.pdf
pZE23MCS colE1 Kanamycin PA1lacO-1 MCS pZE23MCS.pdf
pZE23luc colE1 Kanamycin PA1lacO-1 luciferase pZE23luc.pdf
pZE24MCS colE1 Kanamycin Plac/ara-1 MCS pZE24MCS.pdf
pZE24luc colE1 Kanamycin Plac/ara-1 luciferase pZE24luc.pdf
pZE25luc colE1 Kanamycin PN25tetO-1 luciferase pZE25luc.pdf
pZE31luc colE1 Chloramphenicol PLtetO-1 luciferase pZE31luc.pdf
pZE32luc colE1 Chloramphenicol PLlacO-1 luciferase pZE32luc.pdf
pZE33luc colE1 Chloramphenicol PA1lacO-1 luciferase pZE33luc.pdf
pZE34MCS colE1 Chloramphenicol Plac/ara-1 MCS pZE34MCS.pdf
pZE53luc colE1 Tetracycline PA1lacO-1 luciferase pZE53luc.pdf

-> Overview of available vectors



1.2 Vectors for chromosomal Integration

Vector Origin of replication Resistance Promoter Cloning site
Map/
Sequence (.pdf)

pZS4Int-laci pSC101 Spectinomycin -- -- pZS4Int-laci.pdf
pZS4Int-tetR pSC101 Spectinomycin -- -- pZS4Int-tetR.pdf
pZS4Int-laci/tetR pSC101 Spectinomycin -- -- pZS4Int-laci/tetR.pdf



2 E.coli strains

Strain Genotype Main features

DH5alphaZi

laciq, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1


Its high transformation efficiency makes DH5alpha suited for DNA cloning and DNA preparation. Due to its relatively low growth rates it is not widely used for large scale protein production (e.g. by fermentation)

DH5alphaZ1 laciq, PN25-tetR, SpR, deoR, supE44, Delta(lacZYA-argFV169), Phi80 lacZDeltaM15, hsdR17(rK- mK+), recA1, endA1, gyrA96, thi-1, relA1

C600Z1
laciq, PN25-tetR, SpR, lacY1, leuB6, mcrB+, supE44, thi-1, thr-1, tonA21

A high transformation efficiency combined with extremly fast growth rates makes C600Z1 a superb candidate for DNA cloning as well as high level protein production. RecA mediated chromosomal integration of DNA-sequences and general transduction, e.g. by P1, is also feasible.

C600Zi
laciq, SpR, lacY1, leuB6, mcrB+, supE44, thi-1, thr-1, tonA21

W3110Z1
laciq, PN25-tetR, SpR, IN (rrnD-rrnE)1, rph-1 (see: ATCC 39936)
W3110 is widely used in large scale protein production processes (fermentation). Due to its low transformation efficiency it is not suited for DNA cloning.



3 Kits

Kit Purpose of use System components Main features

pZ Lac
Basic
Standard DNA cloning.

High-level expression of recombinant proteins.

Protein purification.
Cloning and Expression Vectors:

pZE13MCS (pZE13MCS.pdf)

Control Expression Vector:
pZPlac

E. coli strain C600Zi
Vectors contain combinations of the following modules:

• Expression module:
IPTG-inducible promoter PA1lacO-1 in combination with either MCS or luciferase

• Resistance module: Ampicillin

• Replication module: colE1

Host strain C600Zi produces constitutively chromosomally encoded Lac Repressor



pZ Tet
Basic
Standard DNA cloning.

High-level expression of recombinant proteins.

Protein purification.
Cloning and Expression Vectors:

pZE15luc (pZE15luc.pdf)

Control Expression Vector:
pZPtet

E. coli strain C600Z1

Vectors contain com binations of the following modules:

• Expression module:
Tetracycline-inducible promoter PN25tetO-1 in combination with luciferase

• Resistance module: Ampicillin

• Replication module: colE1

Host strain C600Z1 produces constitutively chromosomally encoded Lac Repressor and Tet Repressor



pZ High
Performance
Quantitative and independent control of up to three different transcription units.

Cloning and expression of toxic genes and gene products.

Functional analysis of low copy number proteins where a broad window of regulation (up to 80,000 fold) and/or graded expression is required.

High-level expression of recombinant proteins

Cloning and Expression Vectors:

pZE21MCS (pZE21mcs.pdf)

pZA31MCS (pZA31mcs.pdf)

pZE12luc (pZE12luc.pdf)

pZE53luc (pZE53luc.pdf)

pZS*24MCS (pZS*24mcs.pdf)

Control Expression vectors:
pZPtet and pZPlac

E. coli strains:
DH5alphaZ1
W3110Z1
Vectors contain combinations of the following modules:

• Expression modules:
IPTG-inducible promoters PA1lacO-1 and PLlacO-1
IPTG/Arabinose-inducible promoter Plac/ara-1 ,
Doxycycline-inducible promoter PLtetO-1
combined with either an MCS or luciferase.

• Resistance modules:
Kanamycin
Chloramphenicol
Tetracycline

• Replication modules:
colE1
p15A
pSC101*

Vector modules are largely interchangeable by unique restriction sites and compatible with pZ BASIC.
Host strain DH5alphaZ1 and W3110Z1 produce constitutively chromosomally encoded LacR, TetR, and AraC.



pZ Custom Construction of custom E. coli host strains producing constitutively LacR and/or TetR from chromosomally or episomally located genes.

Vectors for chromosomal integration or episomal maintenance:

pZS4Int-laci (pZS4Int-laci.pdf)

pZS4Int-tetR (pZS4Int-tetR.pdf)

pZS4Int-laci/tetR (pZS4Int-laci/tetR.pdf)

Vectors carry genes laci and tetR under the control of constitutive promoters Placiq and PN25, respectively, either separately (pZS4Int-laci and pZS4Int-tetR) or in combination (pZS4Int-laci/tetR).

• Resistance module: Spectinomycin

• Replication module: pSC101

All vectors can be propagated episomally or can be integrated into the E. coli chromosome by Integrase-mediated recombination using pLDR8 as helper vector.

Please note: pLDR8 is NOT included in the kit but can be obtained e.g. from ATCC, Product Number 77357
Reference: Diederich, L et al. (1992). Plasmid, 28, 1, 14-24



Different combinations of system components are available upon request.