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References


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The following includes selected links and publications describing applications of the pZ Expression System in various fields of basic and applied research. A complete list of citing arcticles can be found at e.g. Google Scholar.

Quantitative control and analysis of gene activities

Basan M et al. (2015). Overflow metabolism in Escherichia coli results from efficient proteome allocation. Nature, 528, 99

Brewster RC et al. (2014). The Transcription Factor Titration Effect Dictates Level of Gene Expression. Cell,156, 6, 1312

Mutalik VK et al. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods, 10, 354

Da Re S,
et al. (2007). Tight Modulation of Escherichia coli Bacterial Biofilm Formation through Controlled Expression of Adhesion Factors. Applied and Environmental Microbiology, 73, 10, 3391

Le TT
et al. (2006). Dynamical Determinants of Drug-Inducible Gene Expression in a Single Bacterium. Biophys J, 90, 9, 3315

Golding I & Cox EC (2004). RNA dynamics in live Escherichia coli cells. Proc Natl Acad Sci USA, 101, 31, 11310

Elowitz MB
et al. (2002). Stochastic Gene Expression in a Single Cell. Science, 297, 5584, 1183


Independent control of gene activities

Rodrigo G
et al. (2012). De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci USA,109, 38, 15271

Chubiz LM & Rao CV (2008). Computational design of orthogonal ribosomes. Nucleic Acids Res, 36, 12, 403

Leandroa J et al. (2006), P Leandro. Co-expression of different subunits of human phenylalanine hydroxylase: Evidence of negative interallelic complementation. Biochimica et Biophysica Acta, 1762, 5, 544

Kapust RB & Waugh DS (2000). Controlled Intracellular Processing of Fusion Proteins by TEV Protease. Protein Expression and Purification, 19, 2, 312


Cloning and Expression of toxic or harmful Gene Products

Fan Y et al. (2016). Identification of a VapBC toxin–antitoxin system in a thermophilic bacterium Thermus thermophilus HB27. Extremophyles 1-9


Kannoly S et al. (2012). Rethinking the evolution of single-stranded RNA (ssRNA) bacteriophages based on genomic sequences and characterizations of two R-plasmid-dependent ssRNA phages, C-1 and Hgal1. J Bacteriol, 194, 18, 5073


Mok WWK et al. (2010). Decoding toxicity: Deducing the sequence requirements of IbsC, a type I toxin in Escherichia coli. J Biol Chem, 285, 41627


You L
et al. (2004). Programmed population control by cell–cell communication and regulated killing. Nature, 428, 868

Grundling A
et al. (2001). Holins kill without warning. Proc Natl Acad Sci USA, 98, 16, 9348-9352


Screening

Genee HJ et al. (2016). Functional mining of transporters using synthetic selections. Nature Chem Biol, 12, 1015


Fleck LE et al. (2014). A Screen for and Validation of Prodrug Antimicrobials. Antimicrob. Agents Chemother, 58:3 1410


Forsberg KJ et al. (2012). The shared antibiotic resistome of soil bacteria and human pathogens. Science, 337, 6098, 1107


Hsiao A et al. (2006). Vibrio cholerae virulence regulator-coordinated evasion of host immunity. Proc Natl Acad Sci USA, 103, 39, 14542

Cabantous S
et al. (2005). Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nature Biotech, 23, 1, 102

Christ D & Winter G (2003). Identification of functional similarities between proteins using directed evolution. Proc Nat Acad Sci USA, 100, 23, 13202


Transcriptional Cascades/Genetic Switches

Rubens JR et al. (2016). Synthetic mixed-signal computation in living cells. Nature Communications, 7

Park H
et al. (2010). Interdependence of behavioural variability and responses to small stimuli in bacteria. Nature, 468, 819

Stricker J
et al. (2008). A fast, robust and tunable synthetic gene oscillator. Nature, 456, 7221, 516

Hooshangi S
et al. (2005). Ultrasensitivity and noise propagation in a synthetic transcriptional cascade. Proc Natl Acad Sci USA, 102, 10, 3581

Kobayashi H
et al. (2004). Programmable cells: Interfacing natural and engineered gene networks. Proc Natl Acad Sci USA, 101, 22, 8414

Guet CC
et al. (2002). Combinatorial Synthesis of Genetic Networks. Science, 296, 5572, 1466


Overexpression

Oliver JWK (2012). Cyanobacterial conversion of carbon dioxide to 2,3-butanediol, Proc Natl Acad Sci USA, 110, 4, 1249

Guillier M & Gottesman S (2006). Remodelling of the Escherichia coli outer membrane by two small regulatory RNAs. Mol Microbiol, 59 (1), 231

Zhao K,
et al. (2005) The Global Transcriptional Response of Escherichia coli to Induced Sigma32 Protein Involves Sigma32 Regulon Activation Followed by inactivation and Degradation of Sigma32 in Vivo. J Biol Chem, 280, 18, 17758

Cabantous S
et al. (2005). Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nature Biotech, 23, 1, 102

Kauth CW
et al. (2003). The Merozoite Surface Protein 1 Complex of Human Malaria Parasite Plasmodium falciparum: Interactions and arrangements of subunits. J Biol Chem, 278, 25, 22257


Expression in hosts other than E. coli

Nyerges A et al. (2015). A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species. Proc Natl Acad Sci USA, 113, 9 , 2502

Lathem WW
et al. (2007). A Plasminogen-Activating Protease Specifically Controls the Development of Primary Pneumonic Plague. Science, 315, 5811, 509

Hsiao A
et al. (2006) Vibrio cholerae virulence regulator-coordinated evasion of host immunity. Proc Natl Acad Sci USA, 103, 39, 14542

Qian F & Pan W (2002). Construction of a tetR-Integrated Salmonella enterica Serovar Typhi CVD908 Strain That Tightly Controls Expression of the Major Merozoite Surface Protein of Plasmodium falciparum for Applications in Human Vaccine Production. Infect Immun, 70, 4, 2029


Reviews

Purcell O
et al. (2010). A comparative analysis of synthetic genetic oscillators. Journal of the Royal Society Interface, 7, 52, 1503

Bertram Rand Hillen W (2008). The application of Tet repressor in prokaryotic gene regulation and expression. Microbial Biotechnol,1 , 1, 2

Saida F
et al. (2006). Expression of Highly Toxic Genes in E. coli: Special Strategies and Genetic Tools, in: Current Protein and Peptide Science, 7, 1, 47, Bentham Science Publishers

McDaniel R & Weiss R (2005). Advances in synthetic biology: on the path from prototypes to applications. Current Opinion in Biotechnology, 16, 476

Kaern M
et al. (2003). The engeneering of gene regulatory networks. Annu Rev Biomed Eng, 5,179