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The pZ expression system developed by Dr. Rolf Lutz & Prof. Hermann Bujard offers tools and reagents for gene expression in prokaryotic hosts*. The system consists of the following components:


1. VECTORS for cloning and expression

A versatile vector system of modular structure offers freely interchangeable functional units:

• 5 tightly and independently controllable promoters

• 5 different selection markers

• 4 origins of replication

• 2 cloning sites


2. E. COLI HOST STRAINS

Various host strains provide chromosomally encoded regulatory proteins in defined concentrations warranting tight repression, full inducibility and genetic stability under all conditions of growth.

*Lutz R & Bujard H (1997). Independent and Tight Regulation of Transcriptional Units in Escherichia Coli Via the LacR/O, the TetR/O and AraC/I1-I2 Regulatory Elements. Nucleic Acids Res, 25, 6, 1203




last update: May 2018